Did some gradient gels 1-2 days ago, placed them wrapped in wet paper and plastic foil at 4°C until today. I added the running buffer and removed the comb right before loading the samples. Samples have enough loading buffer with enough glycerol (worked well all the time), so they did not float on the top of the wells. In 2 of the 3 gels, the samples and also the prestained protein marker did run horizontally into adjacent wells, but at the bottom of the well. I could not figure out why. Never happend before. Can anyone help, please?
Generally this happens due to drying and collapsing of polyacrylamide sieves. You should dip the gels in buffer rather than wrapping in wet paper.
Hi Anne,
I think the leakage was due to improper storage of your gel, so next time cover the comb in the well with cotton wool properly and wet it with water .Then put into a nylon and place in the refrigerator(4oC).When removing your comb from the wells,be careful to avoid the collapse of the well to avoid leakage.
I hope this will you.
All the best!
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