Anne Hofmann

2 Questions 6 Answers 0 Followers

Questions related from Anne Hofmann

Western Blot signals appear white instead of black bands afte ECL - why is that?

I transferred my sample on a 0.2µm NC membrane (semidry blot), incubated with antibody (as usual) and did ECL but I saw some weird, white signals instead of black bands. I was able to detect the...

08 August 2018 3,527 9 View

Why was the loaded sample leaking horizontally at the bottom into adjacent wells of my polyacrylamid gel. Can anyone help, please?

Did some gradient gels 1-2 days ago, placed them wrapped in wet paper and plastic foil at 4°C until today. I added the running buffer and removed the comb right before loading the samples. Samples...

03 March 2018 3,307 2 View