I am planning to build a new Raman system for 785nm laser.
For this company that supplies the microscope recommand us to use the inverted microscope because many other labs use this and works better.
I wonder what is different in Raman signal from the inverted and upright microscope.
Of course, it will get better microscope image in the inverted one
but does this type of microscope also affects the quality of the signal??
Thanks.
Inverted or not is more of a choice not determined by the physics of the signal generation. It is more a choice of practical consideration. In biology, where samples are placed on tranparent glass slides (where appropriated thickness corrected optics are also avaiable), inverted configuration (microscope objectives are below the sample) are quite common. Further, immersion oil or immersion water can directly be dripped on the lens and the sample (with glass slide) nicely dipped into it making a nice seal. This way, you could also use a homogoneous back illumitation from the top for normal widefield microscopy. It feels practical for many transparent samples. However, for intransparent sample, such as common in material science or semiconductor physics, inverted microscopes are not as straight forward in my opinion. As you have also deal with glass slides or find ways to attach/fix/mount your sample. If your microscope objective sits above your sample, it is really convenient to just slide your sample under, place it roughly in the right location and its hold by gravity (or a little piece of tape, so it doesnt move, when you use piezoscanners below the sample).
So, as you can see, this is rather a matter of personal opinion, practically and usability. The physics of scattering is the same - no matter, if the light comes from top or bottom.
I hope that helps,
Michael
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