Hello,
For some time I realised the diploid w303 wt strain I was using was less efficient in the number of transformants I was getting. So I did a small transformation control with two different diploid w303 and an haploid w303 and still I was getting 3-4 transformants for the diploid and 27 for the haploid. 1/9 ratio.
After some research I found this :
"The transformation efficiency of the diploid strain is approximately one-third of the haploid strain for unknown reasons. (One possiblity is that fewer diploid cells are used in the transformation per OD 600 than the haploid. This needs to be tested.)"
But I'm still not sure and so are they.
Anyone has a clue?
Hi there,
Unless you have actually run your transformations with the same number of cells you can't evacaute the hypothesis about the cell number effect.
What is also clear is that diploid and haploid cells are definitely different for adaptation for instance: Article Haploids adapt faster than diploids across a range of environments
Transformation might be just less effective for diploid due to this general fitness issue.
...and a simple test would be to plate a dilution of your competent cells and check the real number of viable competent cells.
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