Why to perform in vitro auto phosphorylation of purified recombinant kinase?

18 January 2017 1 9K Report

Invitro autophosphorylation of purified recombinant kinases?

To determine the activity of the kinase (autophosporylation may be the easiest way to demonstrate your purified recombinant kinase is active), to determine the autophosphorylation sites, to find out how autophosphorylation affects the activity towards other substrates and interactions with other proteins ... depends on the questions you want to answer

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