Can crude enzyme be used for immobiliazation?

Can crude enzyme be utilized as immobilized enzyme for industrial application if the crude enzyme shows high activity?

13 July 2020 8,672 4 View

From where can I get microglia cell line like N9 or BV-2 in India ( preferably in Kolkata)?

From where can I get microglia cell line like N9 or BV-2 in India ( preferably in Kolkata)?

05 November 2019 7,719 2 View

How to conjugate a dye to the N-terminus of poly-glutamic acid peptide?

Hi! I am trying to conjugate a dye in its NHS ester form to the N-terminus of poly-glutamic acid peptide ( which is already available in the form of a white solid). I tried 3 times and am tring...

27 June 2019 8,798 3 View

Is it necessary to purify PCR products before cycle sequencing?

I will purify the cycle sequencing products using EDTA/EtOH precipitation method before loading to 96 well plate.

30 November 2018 6,932 4 View

In case of Box behnken design reduced quartic model, do I need to mention all the insignificant terms in the ANNOVA table and polynomial equation?

When I try to reduce those terms the R2 value reduces. As a result I have many terms whose sum of squares and df values are 0. The p value and all other values fall in range.

22 May 2018 8,088 4 View

How to measure voltage dependent strain of a thin film without using electric field dependent XRD???

because we have not any facility of electric field dependent XRD but all the papers suggested this electric field dependent XRD. If anyone can explore any idea then please help me

15 April 2018 263 2 View

In an XRD data if the peak are same with ICSD data but intensity is not matched with ICSD data then is anything worng???

My XRD data for NdNiO3 is similar with ICSD data but intensity are not matching with ICSD data

11 March 2018 8,823 4 View

How a core flood experiment for conformance control can be designed?

The limitation we are currently having is that cores of length upto 6 inch can only be used in our system and we can not prepare composite cores.

03 September 2017 9,093 2 View

Can zero field cooled MH loop measured after some times (few months) for same magnetic sample at same temperature be different?

17 February 2017 6,694 5 View

PLD Vs RF sputtering?

How PLD and RF sputtering are different over each other ? Or why one should choose PLD over sputtering except depositing complicated oxides (e.g: super conductors or perovskites). It is well known...

12 October 2016 1,464 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Thermal reduction of graphene oxide.?

I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...

23 February 2021 4,155 4 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Which compound is getting oxidized during bulk electrolysis with TFAA?

The experiment is really simple. When I do a bulk electrolysis with 1% TFAA (ACN solution) to get dihydrogen, what is going to be oxidized? (working electrode graphit, counter electrode platinum,...

18 February 2021 4,430 1 View

How to dissolve querceitin in HEPES?

I am trying to do the isothermal titration calorimetry study of quercetin. I want to reach a final concentration of 5 mM quercetin in HEPES or MES buffer with

15 February 2021 2,159 3 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

How to do the calculation for super oxide dimutase assay and how to calculate the R squared value in regression equation for catalase assay?

i have the enzymatic antioxidant assay by following the method of Misra and Fridovich, 1972 for super oxide dimutase and Luck, 1974 for catalase assay can any help me how to the calculation for...

11 February 2021 2,241 3 View

How can I add the metal oxide NP's to the cell lines? I have to make some drug and NP conjuhgates. How much concentration to use? Need some help?

Hi everyone. I am working on metal oxides. I need to make some conjugates of drugs and NP. But I am unsure of what concentrations to make. I have read some articles, but they haven't mentioned...

10 February 2021 3,372 2 View

Are there any thermochromic materials that reflect infrared light (~9000nm) at 10C and transmit infrared light at 25C?

So I know some metal-insulators (ex VO2) have thermochromic infrared-reflective properties, but most of these are insulators at low temperatures and conductive at high temperatures. Some...

07 February 2021 1,770 3 View