Why there is a negative and positive value of intensity at same emission/excitation wavelength in EEM?

27 June 2019 1 661 Report

I start using the spectrofluorometer "Duetta" from Horiba scientific. The result that I have from the emission-excitation matrix seems wrong from the negative and positive value..

Tuhin Khan

Can you please show (plot) one emission spectra where it is negative? There is too many spectra in this particular plot (matrix). Are you performing some kind of background subtraction?

Badges
Science topic

Similar topics
Mathematics
Algebra
Matrix

More Emilie Martel's questions See All

What is the dilution of the plasma to make an Elisa ghrelin with Phoenix pharmaceuticals kit ?

I have to measure the blood concentration of ghrelin with an Elisa. But in the Phoenix Pharmaceuticals kit I ordered, he asks me to dilute the sample without saying how many times. So has anyone...

29 September 2020 3,086 1 View

Bacterial Contamination in Laminar Flow Hood?

Hi there! Brace yourself, this is going to be long winded and contains a year's worth of frustrations. I culture moss, and for the last year, I have been fighting a gram negative bacterial...

29 August 2020 6,492 2 View

What is the 'rescue' of an inhibitory effect (in order to rule out toxicity)?

I am going over a reviewer's comments for my paper and being new to the research world I am not sure what the reviewer means when he/she is asking me whether the inhibitory effect of my product...

10 September 2016 9,255 7 View

Any advice on secondary antibody binding without primary antibodies?

I am new at Western Blotting so unsure what could be going wrong. It looks like my secondary antibody (Cell Signalling Goat Anti-Rabbit-HRP) is binding to the PDVF membrane in the absence of a...

15 December 2015 8,663 7 View

Does anyone know a human UBQLN2 antibody which does not recognize mouse UBQLN2?

I have tried a few antibodies which are supposed to be specific to humans, but they all bind to both species. Thank you.

11 December 2015 7,342 3 View

What is the amount of RNA contained in SCG from P2 mice?

I'm trying to extract RNA from superiori cervical ganglia in P2 mice. I obtained very few RNA, so I'm wondering if it's because the samples are very very small, or if it's due to technical issues....

12 March 2015 1,810 3 View

How to remove coverslips from mounted slides ?

I immunostained whole mesenteries from mouse. I mounted them in Dako Fluorescent Mounting Medium. Now I would need to counterstain them. Does anybody has any idea to remove the coverslips from the...

09 March 2015 9,892 23 View

How to I discover the source of a U6 no-reverse transcriptase control contamination?

Getting contamination in my U6 snRNA NRT control based on my qPCR output. Tried 4 different waters and checked my RNA by bioanalyzer. Any suggestions?

02 February 2015 4,416 3 View

What are the practices and legislation about the distance of trees from buildings, housings in your country?

Dear physicians, architects, lawyers, ... I'm very interested to know which pratices and legislation are currently applied in your country about the distance of planted trees from buildings and...

01 January 1970 2,191 13 View

How to build a correct GAM model with repeated measurement and replicates?

I made an experiment with repeated measurement depending on time scale and I was advised to use GAM model to describe my data. I made an experiment on 12 mesocosms (big tanks) for 6 weeks to test...

01 January 1970 7,295 3 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

How to find a correlation between coordinates in a curvilinear coordinate system?

Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...

28 February 2021 364 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

How can I generate random samples from Bingham distribution in R? How can I construct those symmetric matrices?

in the function rbingham(n,A) in R software how can I construct that A (symmetric) matrix?

24 February 2021 3,528 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View