Multi-group Cross-lag analyses?

Dear all, I conduct cross-lag analyses for perceived parenting from both mothers and fathers, and child outcomes, with three time points in AMOS. With my supervisor, we suspected that there...

23 December 2020 1,009 5 View

Is there any mouse model/strain doesn't require radiation for xenograft study ?

Hi, We don't have radiator on our site, but we want to study human tumor cell line in xenograft mouse model. Does anyone know any mouse strain (besides nude mice) or alternative way to do it ?...

22 January 2020 4,821 1 View

What happens biologicaly when we are confronted with an emergency? why is it important for healthcare profesionnals to train in disaster medecin?

My job consists of elaborating different protocoles and training personnel on how to be efficient in the event of a major crisis. I am currently rewriting my training course and i want to find a...

09 December 2019 6,288 3 View

In general speaking, how long can I keep antibody-labeled sections in wash buffer after the staining of secondary fluorescent antibody?

If the mounting is delayed for a day or two, would the specific binding being greatly reduced?

05 December 2017 4,103 3 View

No classical response of CC2-DMPE/DiSBAC2(3) FRET after depolarization on HEK293 cells

Hi guys, I'm trying to use CC2-DMPE/DiSBAC2(3) FRET pairs to image membrane potential and firstly i just try to show classical increase in FRET ratio(460nm increase, decrease in 580nm) in HEK293...

06 July 2017 6,919 0 View

How to minimize tissue shrinkage in histological processing for stereology?

Stereology methods require tissue to be cut in thick sections (such as 50 or 60um in thickness), but it becomes a challenge for immunohistochemistry, as thick sections can be hard for antibodies...

25 August 2016 6,644 2 View

SDS-PAGE: How to separate 28kDa and 30kDa?

18 August 2015 3,686 8 View

Which basal medium is better for mouse mesenchymal stem cells osteogenesis, high/low glucose DMEM?

I wonder which basal medium is more efficient in inducing osteogenesis of mouse MSCs. High-glucose without FBS supplement is recommended by my lab, yet other reports suggest that low-glucose DMEM...

10 February 2015 7,604 10 View

Can you help me with setup of the old DOS software "Galileo" from Oxford Medilog?

The company no longer exists. The program was used in the 1980s and 1990s for recording clinical EEGs. The setup floppy disc and the manual were lost, and the ISA video card of the original...

12 June 2014 3,135 1 View

Hybridoma cells not secreting antibody

Hi everyone, I am growing the hybridoma cells to get the antibody. My problem is when I tested the antibody by running the SDS gel. I have no expected band at all. I have checked both original...

25 January 2012 2,830 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View