May I ask why the website has been inaccessible recently?

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After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

What does a higher LoD indicate in context of COVID19?

I have seen COVID19 test LoD ranging from 180 to 600000 NAAT Detectable Units/mL So a rapid antigen test with LoD of TCID50 1.13 x10^2 per mL and RT PCR TCID50 1x10^-2 per mL (600 NDU/mL) and a...

28 November 2020 1,238 3 View

Why the acrylic functional group -C=C of FTIR was gone?

Has anyone can tell me. Why the acrylic functional group -C=C ( 1680-1660 cm-1 )of FTIR was gone? This sample was use acrylic and urethane to synthesis. I couldn't see the -C=C signal on my...

17 September 2020 8,186 3 View

KCL, NaCl crystalline, NH4Cl, PEG in MOPS with BSA and Procline 300?

Why do we need all three salts in MOPS buffer? and PEG added to the reagent. NaCl crystalline 1.359 g/L KCl 0.708 g/L Ammonium chloride 0.0063 g/L KCL...

26 April 2020 6,080 4 View

Primer Specificity SARS-COV, 2019nCOV?

I have attached the screen shot. In the table below It compares sequences between COVID-19, SARS-COV and Bat COV Mismatch numbers 11 and 9, meaning that COVID-19 compared to the other two...

30 March 2020 9,084 3 View

Competitive immunoassay prinicple?

A competitive immunoassay technique is used. This involves the reaction of anti-HBc in the sample with hepatitis B core antigen (HBcAg) coated wells. Unbound material is removed by washing....

16 March 2020 9,894 3 View

SAC Well Coating?

The wells are polystyrene coated with streptavidin via a biotin BSA linking layer using the SAC well coating method. What is SAC coating method?

06 March 2020 3,016 3 View

Is there any mouse model/strain doesn't require radiation for xenograft study ?

Hi, We don't have radiator on our site, but we want to study human tumor cell line in xenograft mouse model. Does anyone know any mouse strain (besides nude mice) or alternative way to do it ?...

22 January 2020 4,821 1 View

Human chorionic gonadotropin (hCG) IU units?

WHO has established a reference preparation for hCG (currently the fourth International Standard, with an assigned content of 650 IU/ampoule) and a reference preparation for hCG-beta (currently...

30 December 2019 4,888 3 View

Lingo problem, how to use the optimization result of the LP model in the calculation section?

Suppose we have a linear programming model in the below: Model: Max = 3*x1+5*x2 x1

02 October 2019 8,061 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View