After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

What does a higher LoD indicate in context of COVID19?

I have seen COVID19 test LoD ranging from 180 to 600000 NAAT Detectable Units/mL So a rapid antigen test with LoD of TCID50 1.13 x10^2 per mL and RT PCR TCID50 1x10^-2 per mL (600 NDU/mL) and a...

28 November 2020 1,238 3 View

Why the acrylic functional group -C=C of FTIR was gone?

Has anyone can tell me. Why the acrylic functional group -C=C ( 1680-1660 cm-1 )of FTIR was gone? This sample was use acrylic and urethane to synthesis. I couldn't see the -C=C signal on my...

17 September 2020 8,186 3 View

KCL, NaCl crystalline, NH4Cl, PEG in MOPS with BSA and Procline 300?

Why do we need all three salts in MOPS buffer? and PEG added to the reagent. NaCl crystalline 1.359 g/L KCl 0.708 g/L Ammonium chloride 0.0063 g/L KCL...

26 April 2020 6,080 4 View

Primer Specificity SARS-COV, 2019nCOV?

I have attached the screen shot. In the table below It compares sequences between COVID-19, SARS-COV and Bat COV Mismatch numbers 11 and 9, meaning that COVID-19 compared to the other two...

30 March 2020 9,084 3 View

Competitive immunoassay prinicple?

A competitive immunoassay technique is used. This involves the reaction of anti-HBc in the sample with hepatitis B core antigen (HBcAg) coated wells. Unbound material is removed by washing....

16 March 2020 9,894 3 View

SAC Well Coating?

The wells are polystyrene coated with streptavidin via a biotin BSA linking layer using the SAC well coating method. What is SAC coating method?

06 March 2020 3,016 3 View

Is there any mouse model/strain doesn't require radiation for xenograft study ?

Hi, We don't have radiator on our site, but we want to study human tumor cell line in xenograft mouse model. Does anyone know any mouse strain (besides nude mice) or alternative way to do it ?...

22 January 2020 4,821 1 View

Human chorionic gonadotropin (hCG) IU units?

WHO has established a reference preparation for hCG (currently the fourth International Standard, with an assigned content of 650 IU/ampoule) and a reference preparation for hCG-beta (currently...

30 December 2019 4,888 3 View

Lingo problem, how to use the optimization result of the LP model in the calculation section?

Suppose we have a linear programming model in the below: Model: Max = 3*x1+5*x2 x1

02 October 2019 8,061 3 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Age of the Isimila Prehistoric Site in Tanzania?

Does anyone knows results from the new dating program from the Isimila Prehistoric Site in Tanzania?- of course without to give away too much, before a final publication. The data of ca 260...

03 February 2021 6,864 3 View

What's the main sources for a study of "ethics" in the 1500-1620 period?

I would like experts to provide me with the philosophers of the 1500-1620 period who discussed "ethics" in all or parts of their career. Erasmus, Montaigne, Castiglione, Bruno, Sidney and their...

04 January 2021 7,054 3 View

What is a variabel to analyze empirically or attest a theory of Malthus (Economic Major)?

I have a project to evidence Thomas Robert Malthus theory about a principal of population (Malthusian Spectre/Malthusian Trap). I want to attest, his theory about a relationship of population and...

02 January 2021 8,146 3 View

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01 January 2021 5,831 1 View

Do you know anything about Elena Bokus, the early 20th Century Architect / Architectural Designer?

The available research of early 20th century history of architecture in North Macedonia, suggests that probably the first female architect / architectural designer was Elena Bokus (born in 1893...

23 December 2020 2,039 2 View

Has any research on translation investigated the phenomenon of bilingual authors who produce versions of their books in both their languages?

Can the second-language version, strictly speaking, be considered a translation of the first-language version or is it merely a somewhat looser interpretation? How do such authors approach the...

10 December 2020 5,092 25 View

Where should I be looking for sources on the history of sharecropping in the United States?

08 November 2020 368 9 View