Why the acrylic functional group -C=C of FTIR was gone?

Has anyone can tell me. Why the acrylic functional group -C=C ( 1680-1660 cm-1 )of FTIR was gone? This sample was use acrylic and urethane to synthesis. I couldn't see the -C=C signal on my...

17 September 2020 8,186 3 View

Is there any mouse model/strain doesn't require radiation for xenograft study ?

Hi, We don't have radiator on our site, but we want to study human tumor cell line in xenograft mouse model. Does anyone know any mouse strain (besides nude mice) or alternative way to do it ?...

22 January 2020 4,821 1 View

In general speaking, how long can I keep antibody-labeled sections in wash buffer after the staining of secondary fluorescent antibody?

If the mounting is delayed for a day or two, would the specific binding being greatly reduced?

05 December 2017 4,103 3 View

No classical response of CC2-DMPE/DiSBAC2(3) FRET after depolarization on HEK293 cells

Hi guys, I'm trying to use CC2-DMPE/DiSBAC2(3) FRET pairs to image membrane potential and firstly i just try to show classical increase in FRET ratio(460nm increase, decrease in 580nm) in HEK293...

06 July 2017 6,919 0 View

How to minimize tissue shrinkage in histological processing for stereology?

Stereology methods require tissue to be cut in thick sections (such as 50 or 60um in thickness), but it becomes a challenge for immunohistochemistry, as thick sections can be hard for antibodies...

25 August 2016 6,644 2 View

SDS-PAGE: How to separate 28kDa and 30kDa?

18 August 2015 3,686 8 View

Which basal medium is better for mouse mesenchymal stem cells osteogenesis, high/low glucose DMEM?

I wonder which basal medium is more efficient in inducing osteogenesis of mouse MSCs. High-glucose without FBS supplement is recommended by my lab, yet other reports suggest that low-glucose DMEM...

10 February 2015 7,604 10 View

Can you help me with setup of the old DOS software "Galileo" from Oxford Medilog?

The company no longer exists. The program was used in the 1980s and 1990s for recording clinical EEGs. The setup floppy disc and the manual were lost, and the ISA video card of the original...

12 June 2014 3,135 1 View

Hybridoma cells not secreting antibody

Hi everyone, I am growing the hybridoma cells to get the antibody. My problem is when I tested the antibody by running the SDS gel. I have no expected band at all. I have checked both original...

25 January 2012 2,830 5 View

How does IL-1b get secreted in in vitro inflammasome reconstitution systems?

Hi all, In vitro inflammasome reconstitution system is common in studies involving NLRP3, NLRP1 etc. Some examples of murine and human NLRP3 inflammasome reconstitution is linked...

01 January 1970 6,881 1 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How to eradicate the deteriorated contact resistance in the electrical or electronic devices, like SCR or contactor' pin cable ?

Through years operation, some of the devices' pins connection gradually become worse and finally burn out due to the high temperature at the connection point. We can check out these risk points...

04 February 2021 7,401 5 View

Is it possible to find out the wettability and contact angle of water on the rough or corrugated substrate?

First of all, whether it is possible to identify the presence of water and its amount on the Silicon substrate by using only FT-IR spectroscopy. Can ATR-FTIR spectroscopy find out the wettability...

03 February 2021 9,085 4 View

Could you please interpret the data and SAXS patterns of lipid nanoparticles for us?

Dear Prof. Weiss, Recently, we prepared lipid nanoparticles (composed of phophatidylserine and aluminum ions), and we characterized the particles using SAXS. However, we are unable to interpret...

15 December 2020 4,292 3 View

How can I represent contact with cohesive behavior and friction in abaqus?

Hi, I am trying to represent an experimental test of pull out in abaqus, I have managed to get the detachment behavior (first section of the graph) but the part where the fiber slides does not...

30 November 2020 6,474 3 View

Why does surface roughness enhance (super)hydrophobicity?

Hello, according to Wenzel's model, an increase in surface roughness will also increase the contact angle, but what is the thermodynamic reason for it? Considering the lotus effect, does a...

22 October 2020 9,004 8 View

Is it possible that the hydrophilicity of a scaffold decreases by increasing the concentration of graphene oxide?

The water contact angle results exhibited increased hydrophilicity by increasing the graphene oxide(0 to 0.5%w/v). Still, it occurred oppositely at the concentration of 0.75%w/v even with...

19 October 2020 553 3 View

Why is the q-value in SAXS data 2pi/d and not 1/d?

I am usually working with electron microscopy images of macromolecules, and we use reciprocal distance units [1/Angstrom] for units in Fourier Space. A repeating signal every e.g. 10 Angstrom...

27 September 2020 5,008 1 View