After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

What does a higher LoD indicate in context of COVID19?

I have seen COVID19 test LoD ranging from 180 to 600000 NAAT Detectable Units/mL So a rapid antigen test with LoD of TCID50 1.13 x10^2 per mL and RT PCR TCID50 1x10^-2 per mL (600 NDU/mL) and a...

28 November 2020 1,238 3 View

Why the acrylic functional group -C=C of FTIR was gone?

Has anyone can tell me. Why the acrylic functional group -C=C ( 1680-1660 cm-1 )of FTIR was gone? This sample was use acrylic and urethane to synthesis. I couldn't see the -C=C signal on my...

17 September 2020 8,186 3 View

KCL, NaCl crystalline, NH4Cl, PEG in MOPS with BSA and Procline 300?

Why do we need all three salts in MOPS buffer? and PEG added to the reagent. NaCl crystalline 1.359 g/L KCl 0.708 g/L Ammonium chloride 0.0063 g/L KCL...

26 April 2020 6,080 4 View

Primer Specificity SARS-COV, 2019nCOV?

I have attached the screen shot. In the table below It compares sequences between COVID-19, SARS-COV and Bat COV Mismatch numbers 11 and 9, meaning that COVID-19 compared to the other two...

30 March 2020 9,084 3 View

Competitive immunoassay prinicple?

A competitive immunoassay technique is used. This involves the reaction of anti-HBc in the sample with hepatitis B core antigen (HBcAg) coated wells. Unbound material is removed by washing....

16 March 2020 9,894 3 View

SAC Well Coating?

The wells are polystyrene coated with streptavidin via a biotin BSA linking layer using the SAC well coating method. What is SAC coating method?

06 March 2020 3,016 3 View

Is there any mouse model/strain doesn't require radiation for xenograft study ?

Hi, We don't have radiator on our site, but we want to study human tumor cell line in xenograft mouse model. Does anyone know any mouse strain (besides nude mice) or alternative way to do it ?...

22 January 2020 4,821 1 View

Human chorionic gonadotropin (hCG) IU units?

WHO has established a reference preparation for hCG (currently the fourth International Standard, with an assigned content of 650 IU/ampoule) and a reference preparation for hCG-beta (currently...

30 December 2019 4,888 3 View

Lingo problem, how to use the optimization result of the LP model in the calculation section?

Suppose we have a linear programming model in the below: Model: Max = 3*x1+5*x2 x1

02 October 2019 8,061 3 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Does anyone have a copy of the sTOFHLA?

Peppercorn books is out of business, and that the creator of the test has been dead for over 5 years. I am looking to use this tool to assess literacy in community dwelling seniors as part of my...

25 January 2021 4,061 2 View

I need books or anything else to help me to understand options and others financial derivatives products in english or french?

I am studying at Laval university and one of my course is about options and financial derivatives products , I really have some difficult to understand that course so I am looking for any...

20 January 2021 3,742 3 View

What does a negative real part of impedance indicate in a Nyquist plot?

I am trying to quantify Parylene C's hermetic encapsulation quality using EIS with a 2 electrode configuration (WE/WS and RE/CE). I am simply using two patterned gold layers on glass as my...

12 January 2021 7,399 14 View

In potentiostatic EIS, do we apply the ac voltage signal at the CE or WE ?

In In potentiostatic EIS, we do measure the current at the WE. Do we apply the ac + dc signal on the reference electrode or the counter electrode ? for impedance calculation , do we calculate...

10 January 2021 6,275 2 View

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01 January 2021 5,831 1 View

How to simulate the energy yield of an Organic PV module based on the Temperature effects and the albedo effects?

26 December 2020 4,280 5 View

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I am medical student. I want robbins pathologie fr deutsch pdf . how am I find this book ?

13 December 2020 8,136 3 View

Questionnaires in French and Italian?

Hi to everyone. For my Master's Thesis about ASD in Switzerland I need some questionnaires in French and Italian, as I already have the the German versions. Maybe someone knows if there are...

07 December 2020 3,943 2 View

What are the best websites to find international PHD programs?

As the title implies, I am struggling to find good resources for when it comes to finding international funded research projects outside of the UK within the agricultural and plant sciences....

22 November 2020 2,593 2 View