sometime use one restriction enzyme to cut DNA instead of use two restriction enzymes
Dear all, I am doing a targeted methylation analysis on circulating tumor DNA. After bisulfite conversion, I do Whole Bisulfitome Amplification, since DNA concentration after the bisulfite...
25 September 2022 8,098 0 View
Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?
23 February 2021 4,267 3 View
Hi everyone, We are doing a coculture of cancer cell lines and monocytes (THP-1). As a control, we need to cultivate THP-1 in the context of normal cells. For this experiment, we need to polarize...
16 February 2021 9,725 5 View
Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....
02 February 2021 5,235 2 View
Hi everyone, I am doing a quality control for NGS libraries using bioanalzyer 2100 from Agilent. The machine worked for me for almost two years without any problem, with regular cleaning and...
29 November 2020 1,150 0 View
Hello everyone, I am preparing a manuscript for a journal which indicates in the authors guidelines that the citation and referencing style should look like the following: Citation in the text...
22 September 2020 7,103 6 View
Nowadays there are different types of available access networks which are used by end users to connect to the internet. Thus, the users must be provided with seamless network connectivity to stay...
22 November 2019 3,341 4 View
the difference between XRF & ICP-MS analysis??
30 August 2019 4,656 4 View
Hello everybody, I am currently extracting total RNA including microRNA from serum using miRNeasy Serum/ Plasma kit Catalog Number 217184 from Qiagen. Within the procedure, we add a Spike -In...
13 August 2019 2,335 2 View
I working with pancreatic cancer cell lines PT45P1 and would like to know whether there is a better test rather than wound healing assay to assess the cellular proliferation after and before...
06 August 2019 6,358 9 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...
20 July 2024 1,440 3 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
كيف يساهم انزيم الكاتلاز في عملية التمثيل الضوئي في النباتات؟
09 July 2024 7,512 1 View
ماهو دور انزيم الكاتليز في النباتات؟
09 July 2024 5,824 2 View
what is the protocol for enzyme freeze drying?
09 July 2024 6,464 6 View
Hi We have alpha-amylase enzyme that written 5 units /mg protein on its container. How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?
08 July 2024 2,285 2 View
Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...
29 June 2024 9,688 1 View