Why some protocol for DNA extraction use Phenol?

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Maxim Yu. Sinitsky Popular answer

Dear Oadi,

phenol is used for the separation DNA and proteins during extraction process. It allows to precipitate coagulated proteins in the interface between aqueous and phenol layers.

Best regards,

Maxim

Maxim Yu. Sinitsky

Abhishek Vats

I agree with Maxim Yu. Sinitsky

Phenol and water are immiscible and form two phases a aqueous phase and a phenol phase. Phenol is more dense in which denatured protein partition itself and easily separate when centrifuge.

Nagar D P

Phenol extraction is the commonly method used in DNA extraction which remove proteins from Protein-DNA complex. Phenol is immiscible in water. When we vortex phenol and sample, phenol droplets enter into water and denature the proteins. Thus protein can be separated from DNA.