I have a wild type of tomato and i have trouble with germination, can anyone give suggestion to improve germination. Thanks
You might want to try giving the seeds a period of dry afterripening (at room temperature) and then test them in light at a fairly high temperature, e.g. 25/15 C.
I think for wild tomato it is physiological dormancy you can treated by gebberellic acid
Dear Oadi Matny
- check the viability of seeds by tetrazolium staining.
- if the seeds are viable, after-ripen them and test for germination.
- If they are not germinated, remove the thicker testas of wild-type seeds from the micropyle without damaging the endosperm.
- Treat the seeds with potassium nitrate (0.2%) or GA3 for breaking dormancy, and then test for germination.
Best wishes
Kazem Ghassemi-Golezani
Please have a look at these researchgate links and PDF attachments.
https://www.researchgate.net/publication/231831426_A_classification_system_of_seed_dormancy
https://www.researchgate.net/publication/258522075_Gibberellins_regulate_seed_germination_in_tomato_by_endosperm_weakening_a_study_with_gibberellin-deficient_mutants
https://www.researchgate.net/publication/40201633_The_tomato_seed_as_a_model_to_study_seed_development_and_germination
Good luck
Article A classification system of seed dormancy
Article Gibberellins regulate seed germination in tomato by endosper...
Article The tomato seed as a model to study seed development and germination
You might want to try giving the seeds a period of dry afterripening (at room temperature) and then test them in light at a fairly high temperature, e.g. 25/15 C.
The possibilities was highlighted by the authors above. Good luck. Walter
Besides suggestions above, investigate breaking dormancy of other domesticated and wild seeds. Highest dormancy is after physiological maturity but before harvest (in cereals) to prevent premature sprouting. Lowering temperatures of maturing parent may shorten dormancy--a species survival shock treatment. After drying, consider alternating storage temperature (especially refrigeration), moisture, and solar radiation. Loosening or roughing seedcoat may help. DNA analysis may reveal wild tomato mutants insuring survival through lengthened dormancy. Review details of winter wheat seed dormancy by Jeff Edwards http://wheat.okstate.edu/wheat-management/seeding/PSS-2256.pdf; and J. Derek Bewley's "Seed Germination & Dormancy" research (specifically pg. 1061) http://www.plantcell.org/content/9/7/1055.full.pdf
Good come-back question. Breaking dormancy is likely a bigger issue for you than germination.
Some grass seeds require little burning. You try soaking them for 30 mins to 2 hours
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