This is a guess on my part, but you might exchange the counter-ion on the column from calcium to sodium, changing its selectivity, thus changing what compounds it can resolve.
Please note the different types of columns in the link, it appears that the difference is the counter-ion. https://www.brechbuehler.ch/fileadmin/redacteur/pdf/columns-sampleprep/lc-columns/zhrzx.pdf
This is a guess on my part, but you might exchange the counter-ion on the column from calcium to sodium, changing its selectivity, thus changing what compounds it can resolve.
Please note the different types of columns in the link, it appears that the difference is the counter-ion. https://www.brechbuehler.ch/fileadmin/redacteur/pdf/columns-sampleprep/lc-columns/zhrzx.pdf
Dear Niraj,
As mentioned in Jack's answer if you use sodium phosphate buffer it will compete with the Ca+2 ion in phenomenex Rezex RCM column. Therefore, in this kinds of columns, the buffer to be used should have the same counter ion of the column material. For example, sodium phosphate can be used with RNM-Carbohydrate Na+ (8%) Phase Information, potassium buffers can be used with RKP-Potassium K+ (8%) and calcium buffers can be used with RCM-Monosaccharide Ca+2 (8%) and RCU-USP Sugar Alcohols Ca+2 (8%). For others, please see the following link:
http://www.phenomenex.com/Products/HPLCDetail/Rezex/RCM-Monosaccharide%20Ca2%20(8)
Hoping this will be helpful,
Rafik
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