According to the previous researches, 24-hrs AUC was used for AUC/MIC ratio calculation of vancomycin while it's dose interval is 12 hours. Would you please explain it?
I think that the 24-hour AUC is used to determine the magnitude and nature of the pharmacodynamic (PD) driver by dose fractionation experiments, in which the same total dose is administered in different ways, e.g. one dose at time zero, 2 half-sized doses at 0 and 12 hours, 3 doses of 1/3 size at 8-hour intervals, etc. The efficacy is measured in each case by cfu counting.
Once the magnitude of the PD driver is known, and safety information is collected to determine the maximal tolerated dose, the information can be used to determine how to provide an efficacious dose safely.
As said Men et al., in case of vancomycin "(...) AUC0-24/MIC ratio higher than 400 is regarded as the target of clinical success" I think his publication related to PK/PD of vankomycin answer all your questions:
Article Association between the AUC0-24/MIC Ratio of Vancomycin and ...