In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

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27 April 2020 5,111 25 View

What is the dose required for pentylenetetrazol to induce Epilepsy in mice?

Doses in articles range between 70 -100 mg/kg .I want the answer from someone who has worked empirically. Thank you

10 November 2019 8,086 2 View

What is the procedure of isolating tissue from canine cancer for performing Proteomics and NGS?

we intend to perform the proteomics analysis and NGS of the isolated tissue from canine cancer model and need the protocol for proteomics and NGS of canine cancer model.

09 October 2019 4,627 3 View

What is the procedure of isolating tissue from canine cancer for performing Proteomics and NGS?

we intend to perform the proteomics analysis and NGS of the isolated tissue from canine cancer model and need the protocol for proteomics and NGS of canine cancer model.

06 October 2019 3,516 3 View

Which are the second line treatment drugs shifted to first line of treatment of epilepsy in human?

What are the new drugs supposed to treat epilepsy

08 September 2019 2,951 5 View

What is the best way to identity Pre-ictal stage from multichannel long term EEG sequence , if seizure occurrence interval (ictal stage) is known?

06 July 2019 1,602 2 View

Can anyone help me to identify this tissue?

This image corresponds to a canine heart sample taken on the nearest of the coronary sinus area, at the right auricle. We have suspected of chagasic myocarditis. Could be this area the sinus node?...

01 July 2019 1,235 1 View

How to get data OMI from swath file?

I am a new member. I will do something about SO2 from Aura/OMI data. I download data from website GES DISC It provided two format: Swath file and Grid file. But I don't understand about this data....

11 December 2018 988 0 View

Any experience with canine MKI67 ELISA Kit from Mybiosource?

I'm looking for an ELISA kit to measure KI67 expression in canine cells. The only Kit I found online is this one:...

10 November 2018 4,591 0 View

Is it possible to forecast the exact seizure onset moment instead of "seizure occurrence period (SOP)" ?

Seizure Prediction in patients with epilepsy have been significantly developed in recent years by goal of increase sensitivity as well as specificity and decrease False-Positive/h ratio. although...

10 November 2018 1,942 0 View