Can anyone recommend a service provider for library preparation and 16s sequencing of bacterial samples from animals?

For my project, I want to analyse the bacterial community composition of anal swabs from seals. Thus, I'm searching a reliable service provider for both the library preparation as well as 16s...

10 November 2017 6,437 2 View

How do I convert a SNP genotype table into plink binary PED files?

My raw data is simple: One column of SNP IDs followed by two columns for each locus where 1 is allel A and 2 is allel B. A homozogote would thus have 1 1 or 2 2 in the two first columns and a...

01 February 2015 9,547 14 View

How to calculate the explained variance per factor in a principal axis factor analysis?

I did a principal axis factor analysis with promax (oblique) rotation in R. Now I want to compute the explained variance for all factors of my 4-factor solution. I can extract the eigenvalues but...

10 November 2014 7,829 10 View

What's the best model to predict a categorical outcome with 41 levels (in R)?

Precisely, I want to find out which of the 4 variables can predict membership to the 41 groups (each consisting of just 2 observations).

08 September 2014 8,125 8 View

Can anyone help with R pipeline to calculate Fst measure of population differentiation from AB1 files?

I am a real beginner in bioinformatics and need some hints. My data are mtDNA sequences in the AB1 format. My aim is to compute the Fst to get some insights in the population differentiation...

07 August 2014 6,631 5 View

How can I do Factor Analysis / PCA with more variables than observations in R?

06 July 2014 4,844 13 View

Can anyone suggest a good overview article for the ecology of olfaction?

Especially concerning communication, kin recognition, mate choice?

06 July 2014 510 4 View

How many principal components can I extract from a PCA?

I'm working on seal olfaction and did a PCA on a matrix of 220 scent compounds from 82 individuals. Is there a good way to figure out how many PCs may be relevant? I already did a scree plot and...

05 June 2014 7,958 14 View

What is the best method to align chromatograms/peaks from GCMS?

The chromatograms are saved as Xcalibur files and i want to align them (e.g. via R). They actually have a large variance in peak number / concentration and i need an algorithm to make them...

01 February 2014 3,117 17 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View