Are there any studies or protocols that justify the use of PTFE inserts over other types, such as PET or polycarbonate? I would greatly appreciate any comments or links!
Hello Ilia V. Potapenko,
PTFE inserts are used in organotypic cultures because of its biocompatibility and non-adhesive nature. Being a chemically inert material, it does not react with cells or tissues, ensuring a biocompatible environment. Its non-stick nature prevents the explant from adhering to the insert, allowing for easy manipulation and preventing damage to the tissue. It allows for the maintenance of the 3D structure of the tissue, which is crucial for studying organotypic cultures. Also, the porous nature of PTFE membrane allows for the efficient diffusion of nutrients, oxygen, and waste products across the membrane, supporting the viability of the cultured tissue.
Detailed information on the properties of PTFE may be obtained from the link below.
https://www.sciencedirect.com/topics/chemical-engineering/polytetrafluoroethylene
Best,
Hello
According to this application note (link provided below), PC had the best performance regarding cell attachment followed by PET then PTFE. This might be an advantage in monolayer cell culture or viability assays but not with organotypic cultures.
https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/application-properties-materials-porous-membranes-cell-culture-inserts-application-note.pdf
- Badges
- Science method
- Similar topics
- Biological Science
- Ecology