Ilia V. Potapenko

9 Questions 9 Answers 0 Followers

Questions related from Ilia V. Potapenko

Is DV Coordinate Variability Normal in Biateral Stereotaxic Injections?

Dear colleagues, I’d like to discuss a technical question regarding stereotaxic injections in mice. During bilateral injections, I’ve observed occasional asymmetry in DV coordinates between left...

10 July 2025 2,213 4 View

Why do I see green fluorescence in absence any green fluorophores?

Greetings all! I am seeking help with a question I recently stuck with. In the images below, you can see an example of the immunostaining of brain tissue. There is only DAPI and auto-fluorescence...

05 January 2024 5,203 10 View

Mice behavior after Tamixifen injection: what does it look like?

Mice behavior after Tamoxifen injection: what does it look like? Dear community, I will be very grateful for any clues about the issue. I was stacked with problems regarding mice's behavior...

14 September 2023 7,359 3 View

How to make mice settle down in Von Frey test?

Dear community, I felt in trouble with the Von Frey test that I could not overcome. I have a classic before-and-after experiment with alogene injection into the hindpaw. In about 5 days, I am...

24 July 2023 8,339 0 View

How to distinguish visceral pain related behavior in mice?

Greetings, community! I am mastering visceral pain experiments. We are looking for an easy way to detect visceral pain specific behavior in mice. I have found a couple of articles in which...

19 May 2023 8,489 2 View

How much time does DREAD (hM3Dq or hM4Di) need to be expressed in neurons after targeted recombination in the active population?

I am planning an experiment that utilizes 'targeted recombination in active populations' (TRAP) and involves stereotactically injecting the following viral cocktail in the PFC of BL/6...

13 April 2023 6,749 4 View

Confirmation of DREADD: suspicious cells

Greetings! Recently, I observed images that looked a bit suspicious to me. I have 2 groups of mice. 1) with the [AAV-hSyn-mCherry] virus and 2) with [AAV-hSyn-hM4Di-mCherry] injected in the...

01 January 1970 2,275 0 View

Receptor expression in active neuronal subtypes

Hi, all. I am wondering if there is any chemogenetic or optogenetic AAV that can drive expression of receptors in active neuronal subtypes (e.g., CaMKII+ or GAD+). I know that active populations...

01 January 1970 1,115 0 View

Why PTFE inserts are used for organotypic cultures?

Are there any studies or protocols that justify the use of PTFE inserts over other types, such as PET or polycarbonate? I would greatly appreciate any comments or links!

01 January 1970 6,531 2 View