What is the right time course when using cycloheximide to inhibit protein synthesis?

I'm going to use cycloheximide to inhibit the synthesis of a membrane protein in HEK-293T cells, but I'm not sure the right time that needed to treat cells without influencing its viability. I...

21 September 2020 4,321 2 View

How to get bending moment in Ansys shell181 element?

Hello, I would like to compare the bending moment of a tube with beam and shell element in ansys? The tube has the same dimension in beam and shell element. In beam element, i can get the...

24 August 2020 2,352 1 View

How can suspending cell lines (e.g. THP-1) not attach the culture flask bottom?

As the cell density usually higher than culture medium, I just wonder how can suspending cell lines won't attach the flask bottom?

03 August 2020 9,892 5 View

How to simulate the EQE by SCAPS?

Hello, I simulated solar cell according the experiment, Voc,Jsc,FF and PCE is fine. but the EQE is very different compared with experiment. I don't know how to simulate it? I don't consider the...

02 August 2020 1,236 3 View

How can I detect the presence of antibodies in plasma using electrophoresis?

Can we use PAGE to detect presence of antibodies in plasma? I am planning to characterize antibodies after immunization of mice with whole venom. How can I purify the plasma to get rid of other...

30 July 2020 3,400 1 View

How to simulate the end-to-end delay of mobile network with MATLAB?

I want to simulate the end-to-end delay of a packet from the source to the terminal with MATLAB, and it is better to use 5g mobile communication network.Please help,thanks.

05 July 2020 6,027 4 View

Why do HPLC response area changed differently in standard and samples?

Dear research fellows, I encountered a problem recently and don't know how to explain. I reduced the flow rate in my HPLC method by 25%, the areas in my stds increased by 25% as expected, however...

16 May 2020 1,696 6 View

How Can I Detect if the cultured cells are lysed ?

I want to prove a kind of protein in medium is from exocytosis but not lysed dead cells. I can detect the protein in medium by ELISA(no band in Western blotting), but I'm not sure where does the...

20 January 2020 8,768 4 View

Does anyone know which company's Flag antibody from rabbit source can work well in immunofluorescence?

Does anyone know which company's Flag antibody from rabbit source can work well in immunofluorescence?

19 July 2019 7,658 4 View

What is the meaning of gm in Cadence?

I builded a circuit to simulate NOMS' gm, and found it 16.29 mS. But if I use the formulation learned from class, gm=2*Ids/(vgs-vth). Its result is 27.7 mS. I guess the gm in Cadence is...

10 November 2018 732 0 View

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02 March 2021 3,060 3 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View