hello everyone,
I have a question about my experiment related to preadipocytes differentiation. I am using subcutaneous preadipocytes of cattle, when I passage them in 6 well plates and after 80-90% confluency wash with PBS and add differentiation medium (2% horse serum) and see cell under microscope many cells are washed off, and even during differentiation either i wash cells with PBS or change medium with out wash still some cell aspirate with medium and after adding new medium many cells start floating in the medium, i tried many times and change medium very carefully.
this problem even more severe in 12 well plate
i also found some cell looks strange
kindly answer if anybody know about these issues...
some pictures attached