I'm following a method that calls for "Triethanol amine - imidazole - Ca - Mg - mannitol" for an enzymatic assay (endotoxin assay). I do not understand what mannitol is used for her.
Hi Artem, as Adam said it is probably there to stabilize protein structures by keeping the solution at normal osmolarity. Plus mannitol is not easily metabolized so it will help keep contamination in check.