I can see marker bands on my 1% agarose gel but no sample bands. What's weird is I can see fluorescent in loading wells although no sample bands are visible, seems like my sample was clogged into loading wells. Does anyone meet the same situation and know how to deal with it? My sample is just ~1,500 bp and I'm pretty sure the direction of the current of electrophoresis is correct.
I agree with Hao Gu . One possibility is that you are using too much genomic dna and the pcr is failing due to pcr inhibitors and the protein bound genomic dna is staying in the well. Check the amount ( should be about 50ng) and quality of your dna
Firstly, I think you may need to dilute your DNA before doing the PCR if it is luminescent. Second, make sure you haven't forgotten to add anything to the PCR mix. Thirdly, you need to use a positive control for accurate detection. I think if you take all these things into account, you'll be fine.
Measure the DNA concentration and dilute to 5 ng and you should have it.
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