Shaozheng Sun

4 Questions 3 Answers 0 Followers

Questions related from Shaozheng Sun

How to solve protein aggregation during protein purification?

I expressed the protein(46kDa, PI=9.04) with His tag in E.coli and purified it with cobalt beads. However, when I was using Amicon Ultra 15 mL Centrifugal Filters to concentrate elution flowed...

14 November 2023 2,917 3 View

Why protein cannot be expressed in mammalian cells while constructs are correct?

I made a recombinant plasmid and tested it by colony PCR and double restriction digest, both of them showed the construct was correct but the western blot always cannot show the protein I wanted...

09 October 2023 226 4 View

Why my colony PCR result is not consistent with double restriction digest result?

I did colony PCR (one insert-specific primer and one backbone-specific primer) after transformation and the result showed that the construct was correct, but after that, I did a double restriction...

02 October 2023 9,680 6 View

Why no sample bands in agarose gel but in lading well?

I can see marker bands on my 1% agarose gel but no sample bands. What's weird is I can see fluorescent in loading wells although no sample bands are visible, seems like my sample was clogged into...

18 September 2023 530 3 View