In producing monoclonal antibodies for example. Why is no adjuvant used in the final boost?
I recently developed a few mAbs that are able to neutralize SARS2. By ELISA and WB, I found that they all bind to SARS2 RBD. I wanted to map their RBD epitopes so I ordered pre-coated plates with...
02 June 2024 2,877 2 View
Analysing the fiscal implications of peripheral residential development including case studies
10 March 2024 1,754 0 View
Hi all, I'm starting an arachnological research project with Edge Hill University and have encountered a supply problem with pitfall traps of the required dimensions. The previous supplier has...
08 February 2023 4,685 0 View
I was wondering if someone could clear up the difference between domain decomposition in GROMACS and LAMMPS. As I understand it LAMMPS creates ghost atom copies of atoms in surrounding domains and...
07 February 2023 9,041 0 View
When assessing the contribution platelet concentrates make to coagulation, the above tests are commonly used within the platelet concentrate research field. However, are both nescisary for a...
21 November 2022 4,737 2 View
We're currently planning a pilot study to explore movement behaviour within urban and sub-urban greenspace, as well as the contribution of greenspace-based physical activity to total weekly...
23 November 2021 5,756 11 View
I feel like maybe this question is so easy that it's hard and I keep doubting every choice I make, so I thought I would just finally ask online. I have one dependent variable with three levels:...
03 July 2021 230 5 View
I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...
14 February 2021 3,229 2 View
I need to do some PBMC/WBC FACS analysis. How long can my whole blood be stored in EDTA tubes at 4oC so that the lymphocytes are still good to be stained with a florescently-labeled mAb?
11 February 2021 4,766 3 View
Hi all, I'm going to run Flow Cytometery on ferret PBMCs but can't find an Fc block for ferret PBMCs. Does that even exist? Do I need ferret-specific Fc blocking antibodies or can I use:...
18 January 2021 4,757 5 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I try to understand the ESP mechanism working. What kind of sensors it has, Is it necessary to modify or boost its mechanical ?
15 July 2024 4,772 1 View