Why neutralisation reaction with antibodies against endotoxins ineffective?

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Why Seminomas spread mainly via lymphatic route and NSGCT with Haematogenous route?

What is the reason of such specific orientated route of transmission?

01 January 1970 7,637 1 View

What is the reason of 'alcohal'fastness of Tuberculosis?

Mycolic acid is responsible for acid fastness. Mycolic acid by nature is a lipid rich material which must be washed when bacteria is treated with alcohal. Then what makes it alcohal fast?

01 January 1970 7,141 1 View

Why do Ricketssial rashes spare face,palm,sole? Mechanism behind it?

Ricketssia affect endothelium and due to its damage it causes maculopapular rashes but why they doesn't happen on face,sole,palms?

01 January 1970 9,203 4 View

Why primary viral pneumonia has a predilection for individuals with cardiac diseases, particularly mitral stenosis?

What is the reason of this predilection?

01 January 1970 9,739 2 View

In G6PD deficiency why we talk only about damage to rbc? Other cells should have deficiency of the same enzyme increading oxidative stress to them?

Do G6PD deficiency harmfull for all cells? Why we only talk about rbc when it comes to G6PD deficiency?

01 January 1970 7,837 6 View

What are the Non Collagenous Domains of Collagen?

What are Non collagenous domains of Collagen? And why are they called so?

01 January 1970 1,637 3 View

What are the reasons of prolapse of Facial nerve over stapes?

Facial nerve prolapse reasons?

01 January 1970 1,378 3 View

Where i can find the etymology of drugs in pharmacology? Any site regarding that?

How are names given to drugs? Is there any online site where i can get to know the origin of name of the drugs?

01 January 1970 6,320 5 View

When we say inhibition of enzyme cytochrome p450 what do we really mean?

How do a drug inhibit a metabolising enzyme? Is it due to decreased expression of gene producing enzyme?

01 January 1970 9,693 4 View

No title

Why fungus change its morphology with temprature and how is this really happening?

01 January 1970 2,382 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View