This is my ARMS-PCR result. In principle, this sample only show 2 bands (700bp and 534bp) but here, it shows 1 more band which over 1500bp. I don't know why and how to explain this, I need some help.
Thank you.
Most likely you have multiple alleles/sequence variants in the position your primers target in your sample
See https://www.nature.com/articles/aps201752
Péter Gyarmati The N26(+) sample contains DNA from amenia patient, the IC primer and the 26 NF / Control R primer (normal prime to identify Cd26 mutation). I'm still don't understand why it show that extra band. I have 3 more results and all 3 have this extra band (same position)
Could be the primers are binding elsewhere in the genome. Do your samples have any contamination from microorganisms?
Also looks more like 1600bp to me and there is another faint band at 1300bp. Does the amplicon have any repetitive regions? Have you checked if your primers/amplicons can dimerize?
There are a few possible explanations for the presence of the extra band in your ARMS-PCR result:
To troubleshoot this issue, you could try the following:
It is difficult to determine the cause of the extra band without further information or experimentation, but hopefully these suggestions will help you troubleshoot the issue.
These video playlists might be helpful to you:
https://www.youtube.com/playlist?list=PLzBiAPKi8vONSqjcweFWHMRy13y3484bE
https://www.youtube.com/playlist?list=PLzBiAPKi8vOMheKwb0mu1dcZRaeGUFSlw
https://www.youtube.com/playlist?list=PLzBiAPKi8vOPbE7Db9mKwloe34E8rXXEU
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