07 October 2021 3 9K Report

Hi all,

I have treated the MDA cells with a drug, extracted the RNA by TRIZOL, then synthesized cDNA, which is done correctly, but the Real-Time curves do not look correct! (the following figures). I don't know why the curves aren't overlapping?

I repeat the test, but the results are the same.

Thanks for any help.

figure1. Blue peak is NTC, The rest of the peaks are for a primer in MDA cells treated with different doses

figure2. my positive control (B2M)

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