Hi all,
I have treated the MDA cells with a drug, extracted the RNA by TRIZOL, then synthesized cDNA, which is done correctly, but the Real-Time curves do not look correct! (the following figures). I don't know why the curves aren't overlapping?
I repeat the test, but the results are the same.
Thanks for any help.
figure1. Blue peak is NTC, The rest of the peaks are for a primer in MDA cells treated with different doses
figure2. my positive control (B2M)