I work with HA hemagglutinin recombinant protein, then I want to test my protein activity with the AIV ig rapid test. But it did not produce positive results. I did the refolding process with the method from this journal doi: 10.1016 / j.vaccine.2011.06.014 even though what I used was E.coli BL21 (DE3). then the results of the dialysis I got were almost the same conductivity value differed only 224, then the results of the dialysis I tested on the rapid test, and still got negative results. is there still salt that blocks the interaction between antibodies in the rapid test and rHA? or do I need to go through the purification process first before I try it in a rapid test? I am probably missing something. Would somebody be willing to help me?

Thank you very much in advance,

Rima