My problem is related  with ITS primer .Previously i had no problem but from  fews days bands on 1% agarose gel sometimes  show smears, sometimes multiple bands and sometimes increase in amplicon size. I checked all the  reagents and conitions ,and found when used i use buffer and mgcl2 from others lab (and other reagents own) , i got no problem .But when i use either buffer or mgcl2 of own i got  above problems

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