Why my inactivation of gene through schematic gene deletion method failed?

More Hazrat Bilal's questions See All

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Why my colony PCR results of my recombinant bacterial not showing any results?

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Alejandro Martin

Do you have a positive control for the colony PCR?

Hazrat Bilal

@Alejandro Martin yes positive control pUCSTR with my desired gene.

Hazrat Bilal ,

You mean, you transform S. erythreae with pUCSTR and check the resulting colonies by colony PCR, and this works?

You do have a mock transformation control, right?

yes