Hazrat Bilal

4 Questions 12 Answers 0 Followers

Questions related from Hazrat Bilal

How to confirm shigella spp ?

I have 4 species, by doing its 16srDNA analysis its showing match with shigella spp. after doing its MLST via enterobase plateform it shows me ST of E.coli. how I can know the species type of my...

12 November 2019 8,806 3 View

Why my inactivation of gene through schematic gene deletion method failed?

i am trying to insert 4.3kb DNA fragment (70 ul, 50-100ng/ul) into S. erythreae Protoplast having 70 ul concentration . I am using 200ul PEG3350 for transformation, and culture on R3M media...

26 September 2019 1,329 4 View

How i can confirm my plasmid from single RE and double RE digestion?

I am trying to insert my gene in PcDNA3.0. after plasmid construction I confirm it via sequencing which were all right but when I try to express my gene in HeLa and 7702 cell line it were not...

20 June 2019 5,713 3 View

Why my PCR band size is larger than expected?

I have problem with my PCR amplicon size. The desired size is 309 bp while I am getting band size of 550bp. I even change the primer My. Extract plasmid DNA for 3 times. Run PCR on both master...

26 July 2017 5,823 11 View