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Questions related from Hazrat Bilal
I have 4 species, by doing its 16srDNA analysis its showing match with shigella spp. after doing its MLST via enterobase plateform it shows me ST of E.coli. how I can know the species type of my...
12 November 2019 8,806 3 View
i am trying to insert 4.3kb DNA fragment (70 ul, 50-100ng/ul) into S. erythreae Protoplast having 70 ul concentration . I am using 200ul PEG3350 for transformation, and culture on R3M media...
26 September 2019 1,329 4 View
I am trying to insert my gene in PcDNA3.0. after plasmid construction I confirm it via sequencing which were all right but when I try to express my gene in HeLa and 7702 cell line it were not...
20 June 2019 5,713 3 View
I have problem with my PCR amplicon size. The desired size is 309 bp while I am getting band size of 550bp. I even change the primer My. Extract plasmid DNA for 3 times. Run PCR on both master...
26 July 2017 5,823 11 View