Would you please suggest what can be reason if your control s 16 gene is not producing the expected results in bacterial sample in q PCR? While in same reaction the another gene showed absolutely normal peaks or results..
What can we judge from this behaviour of s 16 gene ?Does it question experiment validation?
Frederic Lepretre
Hi Hira,
if you were used to amplify the 16s and had good results from these primers in the past, could you consider a degration of these ones?
fred
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Hira Shahid
Yes i think that can be one of reason. Should i use those Ct values of this refrence gene any way? to calculate Delta Ct values?
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Frederic Lepretre
Hi Hira
the risk is that ct from your target and ct from your reference are too different to be compared and get reliable results.
fred
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