Hello
I have a problem as you can see on the photo. The pvdf membrane was stained with chromogenic dye and washed with ddh2o2. I'm sure alcohol isn't mixed into the dh2o2. I used the blotting papper instead of the Wb stack, could it have caused the dye to spread? or do you have any other suggestions?
Sudeep Kumar
Did you use BCIP/NBT solution for development of pNPP.
The alkaline phosphatase activity product of BCIP/NBT is insoluble in Water.
If you used a premade BCIP/NBT substrate solution, then probably replacing it with a new one might solve this problem.
Good luck
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Sahra Baran
Thanks your feed back.
No I do not use. I don't even know what they are.
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