Hello
What else can be used instead of homogenizer for RNA isolation from tissue (uterus and ovary) ?
The extraction of RNA may be difficult and inefficient without some type of physical homogenisation. I have collected endometrial punch biopsies and disrupted them using a bead-based homogenisation method with the MP Bio FastPrep instrument. This was followed by extraction of protein and nucleic acids using Trizol.
Why are you looking to avoid homogenisation?
There are many homogenizaiton methods but if the tissue should be used for RNA isolation after the homogenization step, RNA integrity should be preserved. Thus, you should find more gentle methods like bead-based homogenization (bead type/shape can change according to your tissue) or sonicators/ultrasonic homogenizers can help you. Please scan the literature to find the best bead material and shape for your application if you're gonna perform a bead-based method. Do not forget to use trizol-based buffers and RNase free environment.
Good luck!
Thank you for your answers. I want to avoid homogenization because we don't have a homogenizer. So I wonder if we can do RNA isolation with available conditions.
If your tissue is fresh than freeze it in liquid nitrogen. Using sterile cold mortar and pastel(kept on ice) you can powder the frozen tissue on ice. Transfer in a tube. To this powder now you add your lysis buffer(e.g.ALT). It should work.
Bead homogenizers is optimal but if you do not have access to any preparation of tissue powder using liquid nitrogen or acetone will do the job.
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