Why mueller-hinton agar is used in routine antibiotic susceptibility testing?
Hi Dr,Aalla
Mueller Hinton Media contains beef infusion, casamino acids, and starch. ,Beef infusion and casamino acids provide energy and nutrients,The levels of tetracycline and sulfonamide inhibitors, thymidine, thymine, magnesium, and calcium ions, are controlled so as not to interfere with susceptibility testing and to yield good growth.
Thanks
hi
Mueller-Hinton agar is the best medium for routine antibiotic susceptibility testing (AST) because of the following reasons:
1-It shows acceptable batch-to-batch reproducibility for susceptibility testing
2-It supports satisfactory growth of most nonfastidious pathogens
3-It is low in sulfonamide, trimethoprim, and tetracycline inhibitors (i.e. concentration of inhibitors thymidine and thymine is low in MHA)
4-A large body of data and experience has been collected concerning susceptibility tests performed with this medium.
Dear Dr. Aalaa,
Mueller-Hinton has a few properties that make it excellent for antibiotic use. First of all, it is a non-selective, non-differential medium. This means that almost all organisms plated on here will grow. Additionally, it contains starch. Starch is known to absorb toxins released from bacteria, so that they cannot interfere with the antibiotics. Second, it is a loose agar. This allows for better diffusion of the antibiotics than most other plates. A better diffusion leads to a truer zone of inhibition.
Regards
https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/MuellerHintonMed.htm
Dear Dr. Aalaa
Mueller-Hinton agar is the best medium for routine antibiotic susceptibility testing (AST) because it shows acceptable batch-to-batch reproducibility for susceptibility testing, supports satisfactory growth of most nonfastidious pathogens, low in sulfonamide, trimethoprim, and tetracycline inhibitors (i.e. concentration of inhibitors thymidine and thymine is low in MHA) and a large body of data and experience has been collected concerning susceptibility tests performed with this medium. ( https://microbeonline.com )
Mueller Hinton Agar is a non-selective, non-differential and therefore it supports the growth of most non fastidious organism. It contains starch which absorbs bacterial toxins which might interfere with antibiotic susceptibility. it allows batch to batch testing of media. It contains soft agar that permits clear absorption of antibiotics and thus clear zone of inhibitions
Mueller Hinton Agar (MHA) has been selected by the CLSI for several reasons:
i. It demonstrates good batch-to-batch reproducibility for susceptible testing.
ii. It is low in sulfonamide, trimethoprim and tetracycline inhibitors.
iii. It supports the growth of most non-fastidious bacterial pathogens and
iv. Many data and much experience regarding its performance have been recorded.
Can I use Mueller Hinton Agar antimicrobial Test method for examining efficacy of treated fabric samples?
Mueller Hinton agar contain starch that absorb toxin release from bacteria
So they cannot interfere with antibiotics
Also Mueller Hinton better diffusion of antibiotics
It is a loose agar. This allows for better diffusion of the antibiotics than most other plates.
It shows acceptable batch-to-batch reproducibility for susceptibility testing. It supports satisfactory growth of most non-fastidious pathogens.
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