Why mtt assay does not have linear profile?

06 June 2017 0 9K Report

"the assay is not linear over a broad logarithmic cell proliferation range due to the ELISA plate reader" I read that somewhere but I don't understand, why?

Badges
Science method

More Yasin Ilgaz's questions See All

How can I filter on chemical structures?

can I filter on chemical structures with their specialtyfor example; I want to list of both vasodilator and antihypertensive Agents as well as Heterocyclic Compounds, 1-Ring. thanks

03 April 2017 1,447 0 View

Which histological staining method is best for cardiac conduction system?

hi silver impregnation method for nervous tissue of the heart. makes me nervous:) I have no experience. I am not sure; silver impregnation is appropriate method for cardiac conduction system. is...

02 March 2017 3,282 0 View

Cells which they have own basal lamina?

smooth muscle cells, Schwann, adipocyte and perineurial cells have own basal lamina, right? I don't remember the other or find any material about this issue.

11 December 2016 564 1 View

How to find various nanoparticles from europe, in order to use for electron microscopy?

10-100 nm nanoparticles which can be recognizable in transmission electron microscopy

10 November 2016 9,059 0 View

Which parts these vesicular (or dropletic) tissue of daphina magna?

hi everyone, i am working about nanoparticles toxicity in daphnia magna. i have semi-thin and thin sectin from it. i wonder what are these vesicular structures? thanks.

01 January 1970 1,700 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View