Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Why the CHi^2 is close to zero with a bad fitting but the Rp and Rwp are still high?

I am refining my XRD data using in FullProf program. The intensity of the modulated X-ray peaks is almost flat. I tried to adjust the peak intensities but unsuccessful. What is the reason? I guess...

23 February 2021 2,304 4 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

Global Warming effects?

Hi Professionals! Due to Global warming the earth temperature increases (https://climate.nasa.gov/effects/), which generates floods, storms, and tornadoes because of melting of ice glacier, in...

08 February 2021 9,038 8 View

Patients with chronic kidney disease could get COVID-19 vaccine?

04 February 2021 278 4 View

How would I get a "certificate of English approve" ?

I need a "certificate of English approve" for my manuscript to be published in a reputed journal. It should be from a native speaker.

04 February 2021 1,331 3 View

When to use Darcy or Fanning friction factor in frictional pressure drop measurement?

04 February 2021 6,174 3 View

How do you incorporate a study that used non-parametric analysis into a meta-analysis where majority of studies used parametric?

I am calculating Hedge's g for effect sizes, so I am pulling means, SDs, and Ns, from each study. However, one study did a Mann-Whitney U test, and so reports Mean Rank, Sum of Ranks, U, Z, and r...

31 January 2021 1,182 5 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

What is the best pH to grow Lactobacillus rhamnosus?

Hi all, I'm doing some research in the proteins secreted by L rhamnosus and I've been trying to optimise the fermentation conditions to maximise production of these proteins. Without pH control...

08 October 2020 4,647 6 View

Why Lactobacillus acidophillus is sensitive to Vancomycin but L. rhamnosus and casei are intrinsically resistant?

Does it have, anything to do with their fermentation status? I know that in several species of LAB, the terminal d-alanine residue is replaced by d-lactate or d-serine in the muramylpentapeptide,...

19 September 2020 3,242 2 View

Acceptable CV% and SD for results between stool samples in qPCR?

What would be the acceptable CV% and SD for copy number results between stool samples in qPCR (same group)? I am evaluating different bacteria (E.coli, Lactobacillus, etc.) using the absolute...

06 August 2020 8,112 4 View

I am looking for this strain, Lactobacillus acidophilus (strain for minicells production). Please can you help me to find it?

I am a master student, I need this strain for research purposes. Lactobacillus acidophilus [ minicells producing strain (Minicells are generally produced by aberrant cell divisions at...

13 March 2020 3,204 3 View

Why do Lactobacillus spp. not pellet in 4% PFA?

I am currently staining and fixing a range of bacteria for a glycobiologist. I am fixing the bacteria in 4% PFA and then washing with PBS. After fixing Lactobacillus spp. in PFA, it wont pellet. I...

02 February 2020 747 1 View

Why do I get higher copy number from qPCR with phylum specific primer than universal 16s primers?

Hi, My team was trying to profile bacterial phylum composition in food sample using qPCR. We used 16s universal primers and primers specific for certain phylum such as Firmicutes and...

01 February 2020 8,482 3 View

How Red colonies came after Sub culturing ?

Research enigma! Cloning in lactobacillus! After transformation I got complete white colonies but after sub culturing the same , It is starting to see red colonies.No idea from where I got those...

20 January 2020 6,401 3 View

Can someone help me with a plasmid miniprep protocol for L. lactis (Gram positive)?

Hello fellow researchers. I am struggling to find a good plasmid miniprep protocol for Gram-positive bacteria, specifically Lactococcus lactis. I have followed several protocols on PubMed, and I...

31 December 2019 2,036 1 View