How can I connect HC-05 to epoc x ( EEG ) sensor ?

Greetings , my name is Abdul and I have this research on how to move a module car by brain wave . The EEG sensor I have is emotive epoc x , I did the data analysis and am ready to move the car but...

11 January 2021 7,204 1 View

Is it possible to use 100% Ethanol as mobile phase for C18 or any reversed phase columns?

I want to separate 2 compounds on HPLC, and I have made the preliminary studies on TLC using reversed phase plates and 100% ethanol as mobile phase which achieved complete separation. Now I want...

13 August 2020 2,229 3 View

Why trans protons and antiperiplanar protons have higher coupling constants in HNMR?

Why in HNMR the coupling constant of trans protons (typically ~17Hz) is higher than the cis protons (typically ~10Hz)? Also, why the antiperiplanar protons in a cyclohexane derivative have higher...

11 June 2020 4,731 4 View

Can I proceed Heck coupling on a compound in the salt form?

Can I do heck coupling reaction on a compound in salt form without turning it into a free base? As for example doing Heck coupling on p-chloropyridiniumchlroide. I don't want to turn my compound...

19 April 2020 5,593 12 View

Is 0.5 ml of DMSO enough to react my (200mg) Ar-Cl with 1.2 equivalent of primary amine using Ullman reaction?

I'm trying to react Ar-CL compound (200mg) with a primary amine (1.2 equivalent), and I've found in the literature that they're using a very low amount of solvents (like 0.5ml) to proceed the...

17 March 2020 1,016 5 View

Can I proceed N-Boc protection for an organic compound which is in the di-phosphate salt form?

My di-phosphate salt compound contains two 3ry amino groups involved in salt formation and a one 2ry amine group that's not involved in salt formation. I wanna proceed N-Boc protection for this...

15 March 2020 945 3 View

My supervisor told me my question and hypothesis are broad and too obvious, what should I do I already finished writing?

So I am writing about hate speech and chose two case studies from Denmark and France. And what I found is that hate speech is heavily influenced by nationalism but my supervisor told it is broad...

03 March 2020 658 3 View

How can I evaporate Methanol from extract by water bath as an alternative of rotavap ?

I have 10 ml methanol extract and unfortunately, my lab is lack a rotavap how can I evaporate this solvent by water bath

16 January 2020 4,897 2 View

HPLC actinomyceyes active compound ?

I have actinomycetes extraction and I need to detected by HPLC, so I need the protocol of hplc RP-8 chromatographic column

29 December 2019 7,578 3 View

Probelm with expressing hetrologous proteins using Pichia pastoris?

07 December 2019 7,502 6 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View