Why is there such a discrepancy in cleavage (indel %) and transfection efficiency?

08 August 2017 0 9K Report

The cells during transfection is not always synchronized population hence there is a chance that different cells are at different stage and therefore their activity may be different from cell to cell. It is possible that the cells are expressing Cas9 but the cleavage itself may not be caused due to these reasons

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Science method

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