Why is there a discrepancy in the Nanopore LA and NA adapter sequences?

15 December 2023 0 290 Report

This is copied from https://community.nanoporetech.com/technical_documents/chemistry-technical-document/v/chtd_500_v1_reval_07jul2016/adapter-sequences

Ligation Adapter (LA)

Top strand:

5'-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3’

Bottom strand:

5’-GCAATACGTAACTGAACGAAGTACAGG-3’

Native Adapter (NA)

Top strand:

5'-TTTTTTTTCCTGTACTTCGTTCAGT ACGTATTGCT-3'

Bottom strand:

5'-ACGTAACTGAACGAAGTACAGG-3'

'ATTGCT' binds the native barcode adapter. There is a missing T for the top strand of the NA adapter. I have added a space where it should be.

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