What happen with my DNA extraction, why I keep having short fragment?

04 March 2024 1 6K Report

I'm doing DNA extraction from Streptococcus isolates for whole genome sequencing. I'm using DNeasy Blood & Tissue Kits from Qiagen, followed by 96 Rapid Barcoding Nanopore. The result keeps showing very short fragments of DNA (under 2kb). Does anyone know why I have this problem? Is there any tips to increase the length of DNA? Thank you

Chenzhi Shi

I found using Qiagen column could cause DNA/RNA breakage. Using original chemical method would be better for longer DNA rather than using column.

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