How to modify an RNA to make it resistant to nucleases?

Marcos Sánchez Barat @Marcos-Sanchez-Barat

10 January 2024 1 7K Report

I am having problems with the stability of a microRNA against exo/endonucleases in serum. I have seen that there are several modifications to make it more resistant, including modification with phosphothiorates. My question is where and how to place these modifications along the RNA strand and how can they also affect the recognition by intracellular RNase H?

Thank you!

Robert Adolf Brinzer

You could use a T7 self splicing intron to make your RNA circular which would protect against exonucleases.

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