Robert Lu

6 Questions 8 Answers 0 Followers

Questions related from Robert Lu

Why is there a discrepancy in the Nanopore LA and NA adapter sequences?

This is copied from https://community.nanoporetech.com/technical_documents/chemistry-technical-document/v/chtd_500_v1_reval_07jul2016/adapter-sequences Ligation Adapter (LA) Top...

15 December 2023 291 0 View

How can I chemically inactivate Qiagen protease prior to DNase treatment?

I am planning to digest proteins that might be complexed with DNA with Qiagen protease followed by DNase treatment. As it is a serine protease, I'm thinking of using PMSF to chemically inactivate...

08 January 2016 2,702 3 View

What pore size PVDF membrane should I use for 10-100kD range of proteins including Cyt C (12kD)?

For my western blot, all my target proteins are within 10-100kD observed masses. I'm concerned that the lowest is Cyt C at 12kD will transfer through and not stick to membranes with standard...

30 November 2015 2,086 4 View

What is causing my yield loss from washing EV pellets with PBS?

I have previously been attempting to pellet extracellular vesicles (EVs) from 1mL PBS resupension in Beckman 357448 1.5mL polyallomer tubes. However I keep getting DNA yields and Phi29 polymerase...

21 July 2015 2,980 7 View

How do I visualize apoptotic bodies, microvesicles and exosome pellet from centrifugation?

I am fractionating conditioned media and I am performing sequential centrifugation in order to separate out apoptotic bodies, microvesicles and exosomes. I am wondering whether it would be...

10 March 2015 6,420 5 View

Can anyone help me figure out why I have streaking in my Native PAGE?

I ordered recombinant hemagglutinin (HA0 ectodomain) with c-terminal His tags. However I'm getting a lot of streaking in my native gel using Tris-Glycine pH 8.6 and Tris-Acetate 3-8% NuPAGE gel....

23 June 2014 619 10 View