Hi, I want to ask why after restriction digestion DNA concentration is very low when ever extract from gel? What is reason? I extract Vector and my gene of interest with SpeI using 30 ul reaction, after running gel i get good results but DNA extracted from gel give very low concen. I follow this protocol of Restriction Digestion

4 buffer   5 ul

BSA        0.5 ul

SpeI          1ul

vector (133ng/ul) & gene of interest (56 ng/ul)     1 ul

ddH2O             22.5 ul

keep at 37 degree O/N

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