Hi, I want to ask why after restriction digestion DNA concentration is very low when ever extract from gel? What is reason? I extract Vector and my gene of interest with SpeI using 30 ul reaction, after running gel i get good results but DNA extracted from gel give very low concen. I follow this protocol of Restriction Digestion
4 buffer 5 ul
BSA 0.5 ul
SpeI 1ul
vector (133ng/ul) & gene of interest (56 ng/ul) 1 ul
ddH2O 22.5 ul
keep at 37 degree O/N