Hello Bushra Hameed
A standard curve is a curve that may be used to determine the concentration of target protein in the sample.
In ELISA technique, if one must quantify the concentration of an antigen or an antibody in a sample, one must plot a standard curve.
One can plot a standard curve by using the known concentrations of the standard reference antigen against the optical density for each concentration. For this purpose, you may use a series of serial dilution of a standard protein (for instance, BSA) of one known concentration across a range of concentrations near the expected unknown concentration.
Then the unknown concentration of the antigen can be interpolated from the linear part of the standard curve to calculate the amount of antigen present in the sample.
A typical standard curve will have a sigmoidal shape in which the higher concentrations of the standard dilutions will reach a plateau in absorbance. It is the relatively long linear region of the curve that makes the ELISA results accurate and reproducible. So, the calculation of the unknown antigen in the sample is carried out through extrapolating the linear part of the standard curve.
Best.
In ELISA, a standard curve, also known as a calibration curve, is a critical component of the assay used to quantify the concentration of a specific substance, typically an antigen or an antibody, in a sample. It is a graphical representation of the relationship between the optical density (OD) or absorbance of the assay and the known concentrations of a standard or reference substance.
You can find a complete guide on how to make the ELISA Standard Curve on this page: https://www.cusabio.com/m-225.html
- Badges
- Science topic
- Similar topics
- Computer Science
- Program