How to sample data to increase presence of solar panels in the images?

I am working on creating deep neural network on geospatial dataset and wanted to know how to do the sampling so that I can increase the presence of solar panels in the images. I cannot use entire...

25 February 2021 3,936 6 View

Do blood cells die after staining in blood smear analysis ?

This might be a very naive question but I've not been successful in finding sources that explicitly say that the cells are alive post staining. Do Romanowsky stains such as Giemsa, Wright etc....

09 September 2020 2,920 3 View

Is it ok if in measurement model one of the construct having less AVE than 0.5?

In measurement model, one of the construct having AVE 0.4 but the composite reliability is 1 and Chi square = 1.099, GFI = 0.995, CFI = 0.997, RMSEA = 0.022. So, is it OK to go ahead in SEM.

17 July 2020 7,088 4 View

Relative telomere length calculation with Ct values?

10 May 2020 7,398 2 View

In empirical research can we present two different framework?

In SEM, can we present two different models, one bases on theoretical base and other for hypothesis testing? OR In one model we have to present model fit and hypothesis?

03 May 2020 3,441 5 View

Which wavelet transform would be better to use for image classification?

I am trying to use wavelet transform as part of pooling operation in Convolutional Neural Network for Facial emotion classification.As part of this I tried with simple Haar wavelet but it doesn't...

05 February 2020 583 6 View

What is the lower limit for reliably counting cells in a typical hemocytometer ?

I am looking to quantify the counting errors associated with serial dilutions. Few sources mention a lower cap of 25,000 cells per ml - does this sound right ?

27 December 2019 3,714 4 View

Which are the perfect hardware platforms to compute ML-based inference at the network edge?

Edge Computing, IoT, Decentralized Machine Learning, Edge Gateways

12 October 2019 9,940 2 View

How to couple eulerian and lagrangian phases in abaqus?

Hello, I am trying to simulate airbind effect on door slamming. I am unable to couple lagrangian and eulerian sections. Thanks

10 September 2019 7,423 1 View

What are relevant theories that might help me to understand how people value urban open spaces?

Apart from economic values of urban spaces measured using CV and HP, there are other social and ecological benefits offered by urban open spaces. I want to understand whether urban residents...

06 September 2019 278 9 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View