why is it that people usually look at mrna expression rather than protein expression when conducting a time course gene expression analysis?
I am trying to identify bacteria on skin that can utilise lactic acid as carbon source. So i intend to swab skin and then grow bacteria on minimal media supplemented with lactic acid. My question...
11 December 2018 3,432 0 View
Hi, I am hoping to get opinions/input since I am undergrad and I see certain trends that I am highly curious about. I have been doing lab rotation to get an extensive research experience....
09 October 2018 9,760 0 View
I applied some mechanical stresses on cells and discovered that FAK and p38 MAPK are activated by phosphorylation. For the purpose of publishing, i feel that it will be great if i can look for...
03 April 2018 5,791 0 View
Im looking to expose cells to hypoxic conditions in minutes (
02 March 2018 5,548 0 View
hi i need advise on the matter i performed RNA-seq after exposing cells to mechanical stress. I had designed a device to deliver this stress to cells. Looking at the RNA-seq data, i see some...
02 March 2018 5,300 8 View
How is proliferation assessed by manual cell counting? I will be happy if anyone can provide protocol on this. I have been told this method is much useful than indirect assays like CFSE but i...
11 December 2017 2,510 0 View
hi i am not sure if it is alright to analyse my data this way. So i prepared 3 samples of controls, 3 samples of condition at 1hr exposure, 3 samples of condition at 6hr of exposure. i collected...
07 August 2017 7,743 5 View
why when wanting to look at genes that respond at say 1hr (early response), 6hr(intermediate response), 16hr (late response) it is better to look at RNA expression rather than protein? has it got...
07 August 2017 4,608 2 View
Let say if i stimulate a cell at 1 hr and 6hr, the RNA expression upregulated at 1 hr may not be reflected at 6hr, but same protein upregulated at 1 hr will be reflected at 6hr. How do i explain...
06 July 2017 783 0 View
i intend to apply mechanical pressure on cell to look at mechanotransduction pathways. i will be using a SILAC based phosphoproteomics. Can the data also indicate that the cell is undergoing...
05 June 2017 8,936 0 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View